Journal: American Journal of Translational Research
Article Title: miR-370 impacts the biological behavior of lung cancer cells by targeting the SMAD1 signaling pathway
doi:
Figure Lengend Snippet: Targeting association between miR-370 and SMAD1. A. Prediction of targets of miR-370 by Targetscan and miRDB. B. Analysis of SMAD1 expression in NSCLC by TCGA and GTEx. C. Detection of SMAD1 expression in LC cell lines by RT-qPCR and western blot. D. Analysis of the targeting association between miR-370 and SMAD1 by DLR assay. Note: miR: MicroRNA; LC: Lung Cancer; RT-qPCR: Real-time Fluorescence Quantitative Polymerase Chain Reaction; SMAD1: SMAD Family Member 1; DLR: Dual Luciferase Reporter; WT: Wild Type; MUT: Mutant; WB: western blot, ***P<0.001, ****P<0.0001.
Article Snippet: After 48 h of transfection, the luciferase activity was determined through a DLR gene detection kit (Promega, Madison, WI, the States) following the corresponding protocol.
Techniques: Expressing, Quantitative RT-PCR, Western Blot, Fluorescence, Real-time Polymerase Chain Reaction, Luciferase, Mutagenesis